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. 2017 Jul 10;7:4928. doi: 10.1038/s41598-017-05401-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LepR⁺ cells contain Runx2-GFP^low and Runx2-GFP⁻ sub-populations. (A) Representative FACS plots (gated on live CD45⁻Ter119⁻CD31⁻ cells) showing the expression of LepR in Runx2-GFP⁺ stromal populations from 5 week-old Runx2-GFP mice. Left panel showing representative FACS plot of WT control (gated on live CD45⁻Ter119⁻CD31⁻ cells). Black and red lines represent the isotype control and specific antibody against LepR, respectively. n = 3. (B) Representative FACS plots (gated on live cells) showing frequency of Runx2-GFP⁺ population in the CD45⁻Ter119⁻CD31⁻LepR⁺ cell population (right panel) from 5–6 week-old Runx2-GFP mice. Left panel showing negative control for LepR antibody (gated on live cells). Black and red lines represent the WT control and Runx2-GFP mice, respectively (right panel). n = 3. (C and D) Quantification of the frequency (C) and absolute number (D) of Runx2-GFP^low and Runx2-GFP⁻ sub-populations in LepR⁺ cells (CD45⁻Ter119⁻CD31⁻). n = 3. *P < 0.05, **P < 0.01. Data are represented as mean ± SD.