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. 2017 Jul 10;7:4972. doi: 10.1038/s41598-017-05212-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Improved bacterial clearance is not associated with increased AM numbers nor with increased intra-pulmonary neutrophil recruitment in SPC-HAxTCR-HA mice. Mice were oropharyngeally inoculated with ~1.5 × 10⁴ CFU S. pneumoniae TIGR4 or were mock-treated (PBS). At 24 h p.i. mice were sacrificed and phagocytic leukocyte subsets in the BALF (a,b) were quantified by flow cytometry. (c) BALF supernatants were anylyzed by ELISA for levels of complement component C5a. Data were pooled from at least 2 independent experiments (n = 5–8/group). **p < 0.01.