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. 2017 Jul 10;7:5026. doi: 10.1038/s41598-017-04913-0

Figure 3.

Figure 3

Interaction analysis of chickpea MKKs and MAPKs by yeast two-hybrid. Split-ubiquitin based Y2H system was used for checking interactions between 7 CaMKKs and 21 CaMAPKs in 147 possible combinations. The CaMKKs were cloned in pGDHB1 and CaMAPKs in pGPR3-N vector. The plates were photographed after 48 h of yeast growth. Strong protein-protein interactions showed growth on SD/-L-W-H-A media and blue colour after X-gal overlay assay. Weak interactions showed less growth on SD/-L-W-H-A media and light blue colour. Photographs were arranged in an array for comparison and better visualization. Reciprocal interaction assay was also performed.