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. 2017 Jul 10;7:5000. doi: 10.1038/s41598-017-04977-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cultured human islets produce distinct populations of particles. (a) Nanoparticle tracking analysis (NTA) graph of mean particle levels in ICM from ten donors (n = 10) shows variable levels between preparations. (b) NTA histogram of particle size distribution shows the majority of particles detected are between 100 nm and 650 nm. Data are expressed as mean ± SEM of ten donors (n = 10). At least 5 recordings, 30 seconds each were obtained for each sample at 37 °C, camera shutter speed of 30.0 ms, camera level 14 and detection threshold set to 9.