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. 2017 Jul 10;7:4973. doi: 10.1038/s41598-017-05403-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time course analysis of in vitro uptake of ¹⁸F-FDG. Mean ¹⁸F-FDG uptake was determined over a period of 60 min for clinical isolates of S. aureus (red) and E. coli (dark blue), and for the B. subtilis type strain 168 (light blue) and its ptsG (orange) or ptsI mutant (purple) derivatives. Heat-killed bacteria (orange) served as negative controls. Samples were withdrawn at the indicated time points to assess the ¹⁸F-FDG uptake. *p < 0.01 vs. heat-killed controls; ^#p < 0.001 vs. both mutants; Δp < 0.001 vs. the ΔptsG mutant.