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. 2017 Jul 10;7:4943. doi: 10.1038/s41598-017-05213-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PDK1, but not Rictor, plays a dominant role in Akt-mediated HSC functions. (A) Quantification of colony numbers generated by WT, Rictor^Δ/Δ, PDK1^Δ/Δ and Rictor^Δ/Δ PDK1^Δ/Δ BM cells (2 × 10⁴/well) in colony-forming assays. (B) Survival rate of lethally irradiated recipients (CD45.1⁺) transplanted with whole bone marrow cells from WT, Rictor^Δ/Δ, PDK1^Δ/Δ and Rictor^Δ/ΔPDK1^Δ/Δ mice (CD45.2⁺), (n ≥ 10). (C) Whole bone marrow cells from WT, Rictor^Δ/Δ, PDK1^Δ/Δ and Rictor^Δ/ΔPDK1^Δ/Δ mice (CD45.2⁺) that were mixed with WT whole bone marrow competitor cells (CD45.1⁺) in a 1:1 ratio were transplanted into lethally irradiated recipients (CD45.1⁺). Representative FACS plots showing the reconstitution portion of donor cells against competitors in PB. (D) PB chimera rates in recipients 8, 12, 16, 20, and 24 weeks after competitive transplantation. (E,F) The frequencies of donor-derived BM Lin⁻ cells, LSKs, LT-HSCs, ST-HSCs and MPPs in recipients. The data are shown as the mean ± SD (n ≥ 3); *P < 0.05; ***P < 0.001; NS, not significant.