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. 2017 Jul 10;7:4943. doi: 10.1038/s41598-017-05213-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PDK1-deficient HSCs display lower ROS levels than controls. (A,B) LSKs (A) and CD34⁻LSKs (B) from WT or PDK1^Δ/Δ mice were stained with DCF-DA and analyzed using flow cytometry. Representative histograms showed the MFI (median fluorescence intensities) of DCF-DA-labeled cells from WT or PDK1^Δ/Δ mice. (C) BM cells from WT or PDK1^Δ/Δ mice were treated with BSO at various concentrations for colony-forming assays. (D,E) 300 HSCs were sorted from WT or PDK1^Δ/Δ mice and treated with BSO (0.02 μM) for colony-forming assays. The data are shown as the mean ± SD (n = 5); *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant.