Figure 1.

Protein S-mycothiolation pattern and MSH depletion in C. diphtheriae under NaOCl stress. C. diphtheriae was grown in HIB medium to an OD₅₈₀ of 0.75–0.8, transferred to BMM and treated with 300 and 400 µM NaOCl which resulted in growth delay (A) and strongly increased protein S-mycothiolation as revealed by non-reducing MSH-specific Western blots (B). The level of reduced MSH was 0.3 µmol/g rdw in the control and strongly depleted under NaOCl stress in the thiol-metabolome indicating that MSH is used for protein S-mycothiolation (C). All data represent mean values of three independent biological replicates and the error bars given were calculated as standard error of the mean (SEM).