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. 2017 Jul 10;7:5004. doi: 10.1038/s41598-017-05335-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of differences in the input ratio of RNA segments on the outcome of AMV infection. (A) Scheme of the sampling process, with indication of the three leafs sampled. (B) Experimental determinations (by RT-qPCR) of the frequency of each one of the three RNA segments in samples of total RNA for different input ratios. Bars represent the mean of n = 3 plants; error bars represent ±1 SEM. (C) Normalized frequency ternary plot showing the estimated SGF. Solid circles represent the indicated inoculation rates. Open circles show the marginal mean estimates of relative ratios at the end of the experiment corresponding to each input ratio. Lines crossing the open circles represent the 95% Cis. Red arrows connect initial and final ratios. (D) As in (B) but determined for encapsidated RNAs. (E) As in (C) but for encapsidated RNAs.