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. 2017 Jul 10;7:5004. doi: 10.1038/s41598-017-05335-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of the three genomic segments of AMV on transgenic N. benthamiana plants that express viral proteins P1, P2 or both. (A) Experimental determinations (by RT-qPCR) of the frequency of each AMV genomic in total RNA extractions from five different host species. Bars represent the mean of n = 3 plants; error bars represent ±1 SEM. (B) Normalized frequency ternary plot showing the effect of transgenic expression of viral RNAs in the estimated SGF. (C) As in (A) but determined for encapsidated RNAs. (D) As in (B) but for encapsidated RNAs.