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. 2017 Jul 10;7:4957. doi: 10.1038/s41598-017-04506-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of CTCF proteins. (a) Western blotting of CTCF proteins, using an anti-CTCF antibody. Protein extracts from human GM12878 cells, stage 32 chicken embryo, adult lamprey liver, and stage 27 lamprey embryos were used for the analysis. β-actin (ACTB) or histone H3 was used as a loading control protein. (b) Immunoprecipitation. Silver-stained SDS PAGE gel of IP proteins showing chicken CTCF protein of approximately 140 kDa, and lamprey CTCF protein of >250 kDa. Detailed procedures of western blotting and immunoprecipitation are described in Supplementary Materials and Methods. Note that the band positions may not be accurate possibly because of posttranslational modification.