Figure 2.

RNA Foci Formation in SCA36 Patient iPSCs

Fluorescence in situ hybridization (FISH) analysis was performed for each iPSC clone using a cy3-conjugated C(CAGGCC)₂CAG locked nucleic acid oligonucleotide probe. (A) Sense RNA foci were detected in SCA36 patient iPSCs, but not in healthy control iPSCs. Most of the RNA foci were located in nucleus. Scale bar, 10 μm. (B) Graph shows the percentage of RNA-foci-positive cells. ***p < 0.001. Values are expressed as mean ± SEM from n = 3 clones.