Figure 3.

COUP-TFI-Mediated Transcriptional Regulation of the Ins2 Gene

(A and B) MIN6 cells were transfected with the COUP-TFI overexpression vector (A) or siCOUP-TFI (B). (C and D) bmMSCs were transfected with the COUP-TFI overexpression vector (C) or siCOUP-TFI (D). After 72 hr, Ins2 mRNA expression levels were determined by qPCR assay. (E) Expression levels of targets of COUP-TFI were determined by qPCR assay after siRNA knockdown of COUP-TFI in bmMSCs. (F) 3T3 cells were co-transfected with pGL3-Ins2 and phRL-TK in the presence or absence of MafA or siCOUP-TFI, as indicated. The results are expressed relative to the activity observed in the presence of GFP and siControl. Data are presented as means ± SD. Asterisks indicate statistical significance (n = 3, **p < 0.01, ***p < 0.001).