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. 2017 Jul 11;8:1289. doi: 10.3389/fmicb.2017.01289

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Copyright © 2017 Olivares, Badel-Berchoux, Provot, Jaulhac, Prévost, Bernardi and Jehl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prevention of bacterial adhesion by aminoglycosides highlighted by Antibiofilmogram^® results of strains #1 (A) and #24 (B). BioFilm Index (BFI) are expressed as a function of incubation time. This index represents a score of beads aggregation. More precisely, a high BFI value signs no biofilm formation, while a low value shows the presence of sessile cells. Red dotted lines represent control strains (without antibiotic) and continuous curves symbolize adhesion kinetics of bacteria in contact with different concentrations of aminoglycosides for 6 h. The standard deviations represent the mean of six BFI measurements. Statistical analyses have been performed through two-way ANOVA testing and showed that curves with antibiotics are significantly different from the control one at 4 and 6 h of incubation (p < 0.0001). The bead immobilization by bacteria is delayed by antimicrobials and reflects the prevention of the bacterial adhesion.