Fig. 4.

Heterologous OsbHLH068-overexpressing transgenic Arabidopsis plants display a late-flowering phenotype. a Phenotypic comparison of the Atbhlh112 mutant, Col-0, and two 35S::OsbHLH068 transformants grown under LD (left) and SD (right) conditions. Seedlings were grown under LD and SD conditions for 19 and 41 days, respectively. b The bolting time of the Atbhlh112 mutant, Col-0, and two 35S::OsbHLH068 transformants grown under LD (left) and SD (right) conditions. The values are the mean ± SD of two independent experiments, each with ten replicates. *P < 0.05; **P < 0.01, Student’s t-test. c Histochemical staining of OsbHLH068p::GUS transgenic Arabidopsis aerial tissues during the vegetative, transitional, and reproductive stages. Seedlings were grown under LD conditions. Scale bar, 1 cm. Enlarged versions of each black-disc-labeled image are shown in Supplementary Fig. S5a. d Histochemical staining of dark- and LD-treated OsbHLH068p::GUS transgenic aerial tissues over time. Seedlings were grown under LD conditions for 11 days and then transferred to darkness or constant LD conditions for an additional 3 days. Scale bar, 1 cm. Enlarged versions of each black disc-labeled image are shown in Supplementary Fig. S6. Atbhlh112 mutant, SALK_148540