Fig. 5.

Complementary expression of GFP-OsbHLH068 or OsbHLH068-GFP causes a more severe late-flowering phenotype in the Atbhlh112 mutant background grown under LD conditions. a Phenotypic comparison of Col-0, the Atbhlh112 mutant, and the three complemented transformants. The seedlings were grown in soil for 19 days. b The bolting time of Col-0, the Atbhlh112 mutant, and the three complemented transformants. The values are the mean ± SD of two independent experiments, each with ten replicates. *P < 0.05; **P < 0.01, Student’s t-test. c Histochemical staining of the aerial tissues in AtbHLH112p::GUS transgenic seedlings during vegetative, transitional, and reproductive stages. Seedlings were grown in soil for the indicated period of time. Scale bar 1 cm. Enlarged versions of each black disc-labeled image are shown in Supplementary Fig. S8A. d Subcellular localization of GFP-OsbHLH068 (left panel) and OsbHLH068-GFP (right panel) proteins in complemented transformants. Atbhlh112 mutant, SALK_148540