FIGURE 3.

EcN OMVs treatment improves clinical signs of DSS-induced colitis in mice. After the 10-day pre-treatment period, mice received 3% DSS in drinking water for 5 days. Mice were sacrificed 5 days later. Mice from the OMVs-treated group (triangles; n = 9) were administered with EcN OMVs (5 μg/day) throughout the experiment, whereas the two other experimental groups, colitic DSS control (squares; n = 9) and non-colitic control (circles; n = 6), received PBS instead. (A) Weight evolution in animals over the DSS-treatment and the recovery period. Values are presented as percentage of the body weight at the beginning of DSS intake (day 0). (B) DAI score of each experimental group from the beginning of the induction of colitis by DSS treatment (day 0) until sacrifice (day 10), calculated as described in the Section “Materials and Methods.” (C) Colon weight/length ratio calculated following resection. Data are expressed as means ± SEM. Different letters indicate significant differences between groups (P < 0.05).