Figure 5.

The Peyer’s patch (PP) milieu determines B cells trafficking toward PPs. (A) PP lymphocytes (1 × 10⁷) from WT and CCR6^−/− mice were adoptively transferred into recipient mice with different CD45 congenital markers. Recipient mice were sacrificed 48 h post-transfer, and PP lymphocytes were subjected to FACS analysis of donor naive B cells and donor IgA-bearing memory B cells. (B) Concentrations of CXCL13 in PP homogenates from WT and CCR6^−/− mice were determined by ELISA and normalized to the concentrations of total tissue protein. (C) The expression of various genes in PPs of WT and CCR6^−/− mice was determined by quantitative PCR. Relative gene expression was normalized to the level of GAPDH and compared to the expression in WT mice. Each symbol represents one mouse. Data are a compilation of three (A,B) or three to four (C) independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001).