Figure 7.

ILC3s show significantly decreased IL-17 expression but significantly increased MHCII expression in Peyer’s patches (PPs) of CCR6^−/− mice. PP lymphocytes were subjected to immunofluorescence staining of surface markers (Lin, CD45, CD117, CD127, and MHCII) followed by intracellular staining of RORγt. (A) Representative contour plots for the identification of ILC3–LTi (Lin⁻RORγt⁺CD117⁺CD127⁺) in PPs are shown. (B) The frequency of ILC3–LTi in PPs of WT and CCR6^−/− mice is shown. (C) PP lymphocytes were stimulated with 20 µg/ml PMA, 1 µM ionomycin, and 5 µg/ml brefeldin A for 4 h followed by surface staining of ILC3–LTi and intracellular staining of IL-17 and RORγt. The frequency of IL-17-producing ILC3–LTi in PPs of WT and CCR6^−/− mice is shown. (D) The frequency of MHCII-expressing ILC3–LTi in PPs of WT and CCR6^−/− mice is shown. Each symbol represents one mouse. Data are a compilation of six (B), three (C), or four (D) independent experiments (*p < 0.05; **p < 0.01).