Figure 9.

CCR6 deficiency results in the altered intestinal flora. Peyer’s patches (PPs), ileal scrapes, and cecal feces were collected from WT and CCR6^−/− mice. Genomic DNA was purified from the tissues and feces. The levels of indicated bacteria species were analyzed by quantitative PCR using primers specific to Alcaligenes, Bacteroides, Clostridiales, segmented filamentous bacteria, Lactobacillaceae, and Enterobacteriaceae. The quantitative results were normalized to universal 16S rDNA. The levels of indicated bacterial species in PPs (A), ileal scrapes (B), and feces (C) are shown. Each symbol represents one mouse. Data are a compilation of three (A,B) or four (C) independent experiments (*p < 0.05; **p < 0.01).