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. 2016 Nov 30;222(5):2345–2357. doi: 10.1007/s00429-016-1345-3

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Irradiation reduced 2-arachidonoylglycerol (2-AG) levels, but not CB₁ content. a, b Super-resolution images of CB₁s of axon terminals of CB₁BCs were obtained with correlated confocal microscopy and STORM microscopy. Localization points (green dots) in the STORM images represent the position of CB₁s in the axon terminals. There are no changes in CB₁ expression in CB₁BCs as measured by bouton perimeters (c), CB₁ NLP (d), and CB₁ density (NLP/bouton perimeter) (e). Open circles represent mean values of each cell from 12 ± 3 boutons per cell normalized to the mean of control cells. Blue or red filled circles label averages of control and irradiated groups. f There were moderately strong correlations between CB₁ NLP and bouton perimeter in both groups of boutons (n = 107 and 78 boutons from control and irradiated mice, respectively). g Irradiation led to a decrease in 2-AG levels (n = 5 control, n = 4 irradiated)