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. 2017 Jul 11;7:315. doi: 10.3389/fcimb.2017.00315

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Copyright © 2017 Qi, Qin, Du, Chen, Lei, Deng and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of TLR4 and fibroblast growth factor-inducible molecule (Fn14) by porcine small intestinal epithelial cell lines (SIEC02) in response to LPS stimulation. The expression of TLR4 (A) and Fn14 (B) in the SIEC02 in response to the LPS treatment was analyzed by quantitative real-time RT-PCR and Western blot analysis. Representative blots are shown in the upper panel and the histograms in the under panel show the results of densitometric analysis of TLR4 (A) and Fn14 (B) expression (normalized to β-actin to correct for protein loading). The data represent the mean ± SEM (n = 3). ^*P < 0.05, ^**P < 0.01, compared with data on 0-day-old.