Fig. 2.

Altered number and distribution of GABAergic interneurons in the cerebral cortex of semaphorin knockout mice. Images of in situ hybridization for GAD67 (a–c, i–k) and immunohistochemistry for CB (d–f, l–n) in coronal sections through the cortex of wild-type (a, d, i, l), Sema3A ^−/− (b, e, j, m) and Sema3F ^−/− (c, f, k, n) mice at E14.5 (a–f) and E18.5 (i–n). Analysis of the number and distribution of GAD67 labelled cells in all layers of the cortex of Sema3A ^−/− (g,o) and Sema3F ^−/− (h, p) mice at E14.5 (g, h) and E18.5 (o, p). Counts were made in the middle region along the rostro-caudal extent of the cortex. Scale bar in a, i is 100 µm. Error bars indicate SEM (Student’s t test, *p < 0.01, **p < 0.001). CP cortical plate, IZ intermediate zone, MZ marginal zone, SP subplate, SVZ subventricular zone, VZ ventricular zone