Table 2.

Alteration frequency of predictive biomarkers measured by immunohistochemistry and in situ hybridization

Method	Biomarker	Number of samples tested	Number of samples altered	% altered
IHC (high expression)	ALK	123	0	0.0
	AR	648	29	4.5
	BCRP	9	1	11.1
	c‐kit	104	2	1.9
	cMET	538	84	15.6
	COX‐2	10	3	30.0
	EGFR	273	254	93.0
	ER	680	104	15.3
	HER2	700	13	1.9
	MRP1	102	91	89.2
	PD‐1	326	239	73.3
	PD‐L1 (SP142)	368	81	22.0
	PGP	588	11	1.9
	PR	677	23	3.4
	RRM1	596	386	64.8
	SPARC M	506	146	28.9
	SPARC P	546	170	31.1
	TLE3	548	248	45.3
	TOP2A	661	626	94.7
	TOPO1	679	443	65.2
IHC (low/no expression)	ERCC1	296	122	41.2
	MGMT	669	447	66.8
	PTEN	694	412	59.4
	TS	690	460	66.7
	TUBB3	571	138	24.2
ISH (amplification)	c‐MET	421	1	0.2
	c‐MYC	4	1	25.0
	EGFR	85	7	8.2
	HER2	574	13	2.3
	PIK3CA	3	3	100.0
	TOP2A	33	0	0.0