Skip to main content
. 2017 Jun 13;6(7):1639–1651. doi: 10.1002/cam4.1113

Figure 2.

Figure 2

Analysis of MTX and FdUrd induced effects on prosurvival pathways in IGR1 and IGR37 cells. SDS‐PAGE and Western Blot analysis of activation of checkpoint response, DNA repair mechanisms, and expression of proteins involved in de novo and salvage nucleoside synthesis pathways in response to MTX (10 μmol/L, 24 h, lane 2, 5) and FdUrd (10 μmol/L, 24 h, lane 3, 6) in IGR1 and IGR37 cells. Lanes 1 and 4 represent untreated IGR1 and IGR37 cells, respectively. GAPDH served as a loading control (A). Enzyme‐linked immunosorbent assay of GSH concentration in IGR1 and IGR37 cells after treatment with MTX (10 μmol/L, 24 h) and FdUrd (10 μmol/L, 24 h). Data represent means ± SD from three experiments. *P < 0.05 by one‐way ANOVA with Dunnett's multiple comparisons test (B).