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. 2013 Jun 25;305(4):E530–E539. doi: 10.1152/ajpendo.00640.2012

Fig. 3.

Fig. 3.

Genetic inhibition of autophagy prevents adipogenic differentiation. A: Oil Red O staining of 3T3-L1 adipocytes infected with nontarget shRNA or 1 of the 3 Atg5 shRNA lentiviruses. B: quantification of Oil Red O staining [n = 9/treatment; ***P < 0.01 vs. nontarget shRNA (RNAi-N)]. C: Western blot analysis of markers for adipogenic differentiation and transcription factors involved in adipogenic differentiation. D: Oil Red O staining of wild-type (WT), Atg5−/+, and Atg5−/− mouse embryonic fibroblasts (MEF). E: quantification of Oil Red O staining (n = 7–9/genotype; ***P < 0.001 vs. WT). F: Western blot analysis of markers for autophagy, adipogenic differentiation, and transcription factors involved in adipogenic differentiation. Please note that the band indicated by an arrow is Atg5, which is missing in Atg5−/− MEF. All images shown are representative of 3 independent experiments with triplicate samples. FABP4, fatty acid-binding protein 4.