Fig. 2.

Proliferation assay in immortalized undifferentiated human podocytes. Stably nuclear mKate2 expressing wild-type podocytes from an immortalized human podocytes cell line were seeded 12 h before the beginning of the experiment on a 96-well image-lock plate. Images were captured at the beginning and at the end of the experiment. A significant reduction of cell count in average of 23.2% on 240 replicates is shown over a period of 26 h in cultured media containing DMSO vehicle control (0.1%). Data are expressed as means ± SD for 3 independent experiments, 80 replicates each.