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. 2016 Oct 19;312(1):F157–F171. doi: 10.1152/ajprenal.00386.2016

Fig. 4.

Fig. 4.

Proof-of-principle experiment with established modulators of microtubule polymerization/depolymerization such as colcemid, nocodazole, paclitaxel, and vinblastine. Stably nuclear mKate2 expressing wild-type podocytes were seeded 12 h before scratch wound analysis on a 96 well image-lock plate. After scratch wound was made in the confluent podocyte monolayer, podocytes were exposed to compounds including colcemid (no. 10295892001; Roche), nocodazole (M1404; Sigma), paclitaxel (T7402; Sigma), vinblastine (V1377; Sigma), and DMSO vehicle control (0.05%). Concentrations are as follows: 0.05 μg/ml colcemid, 5 μM nocodazole, 5 μM paclitaxel, and 5 μM vinblastine. Colcemid was delivered as ready to use solution, DMSO was added accordingly. Nocodazole, paclitaxel, and vinblastine were diluted in DMSO. All drugs significantly decreased PMR at the established concentrations.