Fig. 5.

Dose response of established modulators of microtubule demonstrates decreased PMR. Stably nuclear mKate2 expressing wild-type podocytes were seeded 12 h before scratch wound analysis on a 96 well image-lock plate. After scratch wound was made in the confluent podocyte monolayer, podocytes were exposed to compounds in different concentration as indicated. A: colcemid (control, n = 47; 0.1 µg/ml colcemid, n = 47; 0.05 µg/ml colcemid, n = 32; and 0.01 µg/ml colcemid, n = 31) alters the PMR partially in a concentration-response manner. B and C: nocodazole (0.1% DMSO vehicle control, n = 45; 10 µM nocodazole, n = 48; 5 µM nocodazole, n = 3; and 1 µM nocodazole, n = 32) and paclitaxel (0.1% DMSO vehicle control, n = 46; 10 µM paclitaxel, n = 48; 5 µM paclitaxel, n = 31, 1 µM paclitaxel, n = 32) reduce PMR in a concentration-independent manner. D: whereas vinblastine (0.1% DMSO vehicle control, n = 47; 10 µM vinblastine, n = 48; 5 µM vinblastine, n = 32; and 1 µM vinblastine, n = 32) reduces PMR in a concentration-dependent manner.