Table 2.
Confirmed positive hits by PMR assay from small molecule screen of 725 compounds from NIHCC with decreased PMR
| Name | Result on PMR | Mechanism | Pubchem ID | Reference | Drug Library Plate | Concentration, µM |
|---|---|---|---|---|---|---|
| Topotecan (Top) | Decreased migration | Anti-neoplastic topo I inhibitor | 46386667 | (64, 99) | NIHCC Plate_1_A8 | 10, 5, 1 |
| Vincristine (VA) | Decreased migration | Vinca-alkaloid-inhibitor of mitosis | 46386588 | (57, 87) | NIHCC Plate_1_G8 | 10, 5, 1 |
| Vindesine (VA) | Decreased migration | Vinca-alkaloid-inhibitor of mitosis | 46386586 | (87) | NIHCC Plate_1_H11 | 10, 5, 1 |
| Digoxin (Dig) | Decreased migration | Na-K-ATPase inhibitor | 104170057 | (1) | NIHCC Plate_8_G5 | 10, 5, 1 |
| Podofilox (Top/TI) | Decreased migration | Tubulin-alpha/beta chain polymerization inhibition, DNA-topoisomerase-2 inhibitor | 104170173 | (54, 59) | NIHCC Plate_9_C8 | 10, 5, 1 |
| Albendazole (TI) | Decreased migration | Tubulin-alpha/beta chain polymerization inhibition | 104170113 | (23, 92, 105) | NIHCC Plate_7_C9 | 10, 5 |
| Lomerizine DiHCl (CaB) | Decreased migration | Ca channel blocker | 46386707 | (45, 94) | NIHCC Plate_4_A4 | 10 |
| Pancuronium (MR) | Decreased migration | Nondepolarizing muscle relaxant; competitive acetylcholine antagonist at NMJ | 46386853 | (29) | NIHCC Plate_4_B3 | 10 |
| Trazodone hydrochloride (SSRI) | Decreased migration | Antidepressant; binds 5HT2; selective reuptake inhibitor | 46386915 | (73) | NIHCC Plate_4_B8 | 10 |
| Tegaserod maleate (5HT) | Decreased migration | 5HT4 agonist; used for IBS | 46386624 | (15) | NIHCC Plate_4_C7 | 10 |
| Irinotecan HCl (Top) | Decreased migration | Antineoplastic-topo I inhibitor | 46386616 | (18) | NIHCC Plate_4_D10 | 10 |
| Mobendazole (TI) | Decreased migration | Tubulin-alpha/beta chain polymerization inhibition | 104170137 | (72, 85) | NIHCC Plate_8_A8 | 10 |
| Mitoxantrone (Top) | Decreased migration | DNA intercalation; DNA-topoisomerase-2 inhibitor | 104170122 | (21, 44) | NIHCC Plate_9_E5 | 5, 1 |
| Adenosine, N-(2-hydroxycyclopentyl)-,(1S-trans)-(AR) | Decreased migration | Activation of purine receptors A1 and A2 | 46387015 | (110) | NIHCC Plate_2_A6 | 5 |
| N,N′-diacetyl-1,6-diaminohexane (Syn) | Decreased migration | experimental compound; used in production of nylon | 46386869 | N/A | NIHCC Plate_2_B2 | 5 |
| Zolmitriptan (Trip) | Decreased migration | Triptan-selective agonist of serotinin 1B and 1D receptor | 46386880 | (74, 89) | NIHCC Plate_2_E11 | 5 |
| Vinorelbine bitatrate (VA) | Decreased migration | Vinca-alkaloid-inhibitor of mitosis | 46386815 | (57, 87) | NIHCC Plate_4_E4 | 5 |
| Artesunate (AM) | Decreased migration | Semi-synthetic derivative of artemisinin | 46386645 | (52) | NIHCC Plate_2_H6 | 1 |
Sixty-one preliminary positive results (see Table 1) were screened in serial concentrations of 1, 5, and 10 µM. Confirmed results are above with respective concentrations at which the result was confirmed. All positive hits were screened in quadruplicate at their respective concentration and compared with a vehicle control (0.01, 0.05, and 0.1% DMSO, respectively). NIHCC, National Institutes of Health Clinical Collection; PMR, podocyte migration rate; AM, anti-microbial; AR, antiarrhythmic; CaB, calcium channel blocker; Dig, digitalis glycoside; IBS, irritable bowel syndrome; MR, muscle relaxant; NMJ, neuromuscular junction; Pubchem ID: https://pubchem.ncbi.nlm.nih.gov/; SSRI, selective serotonin reuptake inhibitor; Syn, synthetic compound; Top, topoisomerase inhibitor; Trip, triptan agonist; TI, tubulin inhibitor; VA, vinca-alkaloid.