Fig. 2.

Short-term (4-h) effects of cyclosporine A on the distal Na-K-Cl cotransporters and potassium channels [renal outer medullary K channel (ROMK) and ATP-sensitive inward-rectifier K channel (Kir4.1)]. A: representative immunoblots of kidney lysates from vehicle-treated and cyclosporine A-treated rats showing immunoreactive signals for NKCC2, pNKCC2, NCC, pNCC (all ~160 kDa), ROMK (~45 kDa), and Kir4.1 (~40 kDa); β-actin served as loading control (~40 kDa). B: graphs showing densitometric evaluation of immunoreactive signals (A) normalized to loading controls. Data are means ± SE; *P < 0.05.