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Long-term (14-days) effects of cyclosporine A on mRNA expression levels of the distal Na-K-Cl cotransporters, WNK-SPAK/OSR1 kinases, and COX-2. Graphs show results of quantitative PCR evaluation of WNK1, WNK3, SPAK, OSR1, NKCC2, NCC, and COX-2 mRNA levels in kidney lysates from rats treated with vehicle or cyclosporine A for 14 days; all results were normalized to GAPDH expression. Data are means ± SE; *P < 0.05.
