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. 2016 Dec 21;312(3):F489–F501. doi: 10.1152/ajprenal.00575.2016

Fig. 12.

Fig. 12.

Short-term (4-h) effects of cyclosporine A on phosphorylation levels of the distal Na-K-Cl cotransporters and SPAK/OSR1 kinases in vasopressin-deficient Brattleboro rats and in cultured cells. A: representative immunoblots of kidney lysates from vehicle-treated and cyclosporine A-treated (4 h) Brattleboro rats showing immunoreactive signals for pNKCC2 and pNCC (both ~160 kDa); GAPDH served as loading control (~40 kDa). B: densitometric evaluation of immunoreactive signals (A) normalized to loading controls. C: representative immunoblots of cell lysates from vehicle- and cyclosporine A-treated DCT cells showing immunoreactive signals for pNCC (~160 kDa) and pS-SPAK/OSR1 (~60 kDa); GAPDH served as loading control (~40 kDa). D: representative immunoblots of cell lysates from vehicle- and cyclosporine A-treated TAL cells showing immunoreactive signals for pNKCC2 (~160 kDa) and pS-SPAK/OSR1 (~60 kDa); β-actin served as loading control (~40 kDa). E: densitometric evaluation of immunoreactive signals (C and D) normalized to loading controls. Data are means ± SE; *P < 0.05.