Skip to main content
. 2016 Dec 21;312(3):F489–F501. doi: 10.1152/ajprenal.00575.2016

Fig. 13.

Fig. 13.

Short-term (4-h) effects of cyclosporine A on phosphorylation levels of NKCC2 and SPAK/OSR1 kinases in cultured TAL cells stimulated by desmopressin (DDAVP). A: representative immunoblots of cell lysates from TAL cells treated with vehicle, DDAVP, or DDAVP and cyclosporine A simultaneously showing immunoreactive signals for pNKCC2 (~160 kDa) and pS-SPAK/OSR1 (~60 kDa); GAPDH served as loading control (~40 kDa). B: densitometric evaluation of immunoreactive signals normalized to loading controls. Data are means ± SE; *P < 0.05 for DDAVP or DDAVP + cyclosporine A vs. vehicle; #P < 0.05 for DDAVP + cyclosporine A vs. DDAVP.