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O-glycosylation profiles of N. benthamiana (NB) and HEK293-6E (HEK)-derived recombinant IgA1. Mass spectra of the hinge region peptide (HYTNPSQDVTVPCPVPSTPPTPSPSTPPTPSPSCCHPR) are shown ([M+3H]3+ for NB and [M+4H]4+ for HEK). Glycosylated peaks are indicated: pentoses (Pent), hydroxyproline (Hyp), putative N-acetylgalactosamine (HexNAc), hexoses (Hex), and sialic acid residues (NeuAc).
