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. 2017 May 18;16(7):2457–2471. doi: 10.1021/acs.jproteome.7b00068

Figure 1.

Figure 1

In vivo cross-linking in of B. subtilis in culture. (a) Growth curves of B. subtilis in minimal medium with 1.2 mM glutamine (filled diamonds) and 5 mM glutamine (open squares). (b) SDS-PAGE analysis of in vivo cross-linking with BAMG and DSG of exponentially growing B. subtilis directly in the growth medium. Control, soluble proteins from untreated exponentially growing B. subtilis. 2 mM BAMG or DSG, soluble proteins from exponentially growing B. subtilis treated with 2 mM BAMG or DSG, respectively. Molecular weights (kDa) are shown on the left-hand side adjacent to prestained molecular weight markers (MW markers).