Table 1.
Outcome of mosquito-feeding experiments
| Exp. number | Patient blood smear (% of RBCs) | Mosquito Oocysts | Mosquito Sporozoites | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P. vivax | Gametocytes | No. dissected | No. Pos. | Oocyst range | Av. oocyst no. | Infect. rate (%) | No. dissected | No. pos. | Infec. rate (%) | Gland index | ||
| Male | Female | |||||||||||
| 1 | 1.35 | 0.31 | 0.25 | 23 | 14 | 0–13 | 2.5 | 61 | 5 | 2 | 40 | 3+ (2) |
| 2 | 0.83 | 0.07 | 0.08 | 20 | 15 | 0–180 | 44.5 | 75 | 14 | 13 | 93 | 4+ (3), 3+ (10) |
| 3 | 0.11 | 0.01 | 0.008 | 20 | 16 | 0–10 | 3.2 | 80 | 21 | 16 | 76 | 3+ (7), 2+ (8), 1+ (1) |
| 4 | 0.07 | 0 | 0.02 | 20 | 2 | 0–1 | 0.1 | 10 | 20 | 0 | 0 | NA |
| 5 | 0.26 | 0.01 | 0.03 | 32 | 31 | 0–68 | 35 | 97 | 20 | 20 | 100 | 4+ (7), 3+ (12), 2+ (1) |
| 6 | 0.28 | 0.04 | 0.03 | 16 | 13 | 0–17 | 4 | 81 | 21 | 14 | 66 | 3+ (8), 2+ (5), 1+ (1) |
| 7 | 1 | 0.22 | 0.22 | 20 | 10 | 0–80 | 23 | 50 | 20 | 17 | 85 | 4+ (3), 3+ (13), 2+ (1) |
| 8 | 0.69 | 0.14 | 0.09 | 20 | 0 | 0 | 0 | 0 | 19 | 0 | 0 | NA |
| 9 | 1.45 | 0.39 | 0.27 | 20 | 19 | 0–218 | 96.5 | 95 | 20 | 20 | 100 | 4+ (2), 3+ (18) |
| 10 | 0.34 | 0.04 | 0.03 | 20 | 14 | 0–18 | 6 | 70 | 20 | 18 | 90 | 3+ (15), 2+ (2), 1+ (1) |
| 11 | 0.35 | 0.03 | 0.08 | 20 | 2 | 0–2 | 0.15 | 10 | 20 | 3 | 15 | 3+ (1), 2+ (2) |
| 12 | 1.03 | 0.15 | 0.12 | 20 | 18 | 0–62 | 14.9 | 90 | 20 | 19 | 95 | 3+ (15), 2+ (3), 1+ (1) |
| 13 | 0.39 | 0.06 | 0.07 | 20 | 16 | 0–22 | 8 | 80 | ND | NA | NA | NA |
| 14 | 0.49 | 0.04 | 0.12 | 20 | 6 | 0–5 | 0.7 | 30 | ND | NA | NA | NA |
| 15 | 0.34 | 0.04 | 0.03 | 10 | 9 | 0–87 | 39 | 90 | ND | NA | NA | NA |
| 16 | 0.55 | 0.04 | 0.05 | 20 | 20 | 4–195 | 66 | 100 | 22 | 21 | 95 | 4+ (13), 3+ (8) |
| 17 | 0.67 | 0.01 | 0.02 | 34 | 16 | 0–83 | 4.2 | 47 | ND | NA | NA | NA |
| 18 | 0.33 | 0.05 | 0.08 | 25 | 22 | 0–15 | 4.9 | 88 | 20 | 11 | 55 | 4+ (1), 3+ (7), 2+ (2), 1+ (1) |
| 19 | 1.21 | 0.25 | 0.33 | 20 | 17 | 0–215 | 95.8 | 85 | 20 | 18 | 90 | 4+ (3), 3+ (14), 1+ (1) |
| 20 | 0.79 | 0.07 | 0.16 | 20 | 18 | 0–40 | 15.7 | 90 | 21 | 19 | 90 | 4+ (3), 3+ (15), 1+ (1) |
| 21 | 0.58 | 0.07 | 0.11 | 20 | 17 | 0–185 | 79.2 | 85 | 20 | 17 | 85 | 4+ (7), 3+ (9), 2+ (1) |
| 22 | 0.3 | 0.01 | 0.03 | 20 | 18 | 0–22 | 7.6 | 90 | 20 | 17 | 85 | 4+ (2), 3+ (7), 2+ (7), 1+(1) |
| 23 | 0.54 | 0.05 | 0.09 | 20 | 16 | 0–50 | 10.5 | 80 | 21 | 15 | 71 | 3+ (8), 2+ (7) |
| 24 | 1.32 | 0.07 | 0.24 | 20 | 04 | 0–1 | 0.2 | 20 | 12 | 0 | 0 | NA |
| 25 | 1.05 | 0.09 | 0.1 | 21 | 02 | 0–2 | 0.1 | 10 | 20 | 0 | 0 | NA |
| 26 | 0.75 | 0.07 | 0.08 | 20 | 7 | 0–4 | 0.7 | 35 | 11 | 2 | 18 | 3+ (1), 2+ (1) |
| 27 | 0.63 | 0.08 | 0.07 | 18 | 13 | 0–8 | 2.5 | 72 | 20 | 9 | 45 | 3+ (2), 2+ (5), 1+ (2) |
| 28 | 0.35 | 0.03 | 0.03 | 20 | 6 | 0–2 | 0.3 | 30 | 20 | 5 | 25 | 3+ (1), 2+ (4) |
| 29 | 0.45 | 0.1 | 0.11 | 14 | 7 | 0–4 | 1.2 | 50 | ND | NA | NA | NA |
| 30 | 0.23 | 0.01 | 0.01 | 27 | 7 | 0–3 | 0.3 | 26 | ND | NA | NA | NA |
Only experiments where at least 10 mosquitoes were dissected for salivary gland infections were used for correlating sporozoite infection rate with parasitaemia and gametocytaemia. Based on this criterion, experiments 1, 13, 14, 15, 17, 29 and 30 were not included for analysis
ND not done, NA not applicable