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. 2017 Jul 11;16:117. doi: 10.1186/s12934-017-0730-9

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Relative transcript levels of selected marker genes for sulfur-, phosphorus-, and nitrogen limitation in the reference and supplemented cultivations (1× SO4, 2× SO₄, 1× PO₄). Transcript levels are normalized to ACT1 housekeeping gene and transcript levels of the middle (27 h) and end of the methanol fed-batch (67 h) are further normalized to the glycerol fed-batch (FB)