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. Author manuscript; available in PMC: 2017 Jul 11.
Published in final edited form as: Virology. 2017 Mar 1;505:148–154. doi: 10.1016/j.virol.2017.02.013

Fig. 4.

Fig. 4

Deletions within the BART locus decrease the accumulation of BART miRNAs. (A) The plasmid 3829 and its deleted derivatives, 4045 and 4046, are depicted. Plasmid 4045 lacks OriLyt and plasmid 4046 lacks one-half of the downstream portion. (B) 1×105 AGS-3829, AGS-4045, AGS-4046 cells were injected in parallel into mice to induce tumors. The tumors were collected and total RNA and DNA were isolated and analyzed as in Figs. 1 and 3. The level of BART3 which is expressed by all of these plasmids was measured by stem loop real-time PCR, and then normalized to the number of EBV genome. The miRNA expression per genome in at least two different clones was measured in more than three independent experiments.