The relative expression of neuronal synaptic proteins GLT1, PSD-95, and SNAP-25 was measured after 3 days of ACM addition to SH-SY5Y and SH-SY5Y-APP cells using Western blot analysis. (A) Representative Western blots and densitometry analysis showed ACM from Aβo and combination treated astrocytes to significantly increase GLT1, PSD-95, and SNAP-25 expressions in SH-SY5Y cells. Oleocanthal treatment has no effect on their expression levels. (B) Representative Western blots and densitometry analysis showed ACM from Aβo, oleocanthal and the combination treated astrocytes have no effect on GLT1 and PSD-95 expressions in SH-SY5Y-APP cells. Only the expression of SNAP-25 was significantly increased by ACM from Aβo and combination treatments. Data is presented as mean ± SD (**P<0.01, ***P<0.001), n= 3 independent experiments.