Figure 2. Tregs are required for hair regeneration.

Foxp3^DTR mice or control WT mice were treated with DT on days −2, −1, and depilated on day 0 to induce anagen. DT administration was continued from Day 1 and then every other day until the termination of the experiment at day 14. (A) Representative photos and (B) kinetics of hair regrowth in WT and Treg depleted (Foxp3^DTR) mice (n = 3–4 mice per group). Tregs were depleted either up to day 4 (early), from day 7 onwards (late) or constitutively (con) throughout the experimental period. (C) Hair regrowth at day 14 in WT and Foxp3^DTR mice with DT treatments as indicated. (D), Representative H&E staining of skin from WT and Foxp3^DTR mice on day 14. Arrows indicate anagen HF extension into dermal adipose. Scale Bars, 100 µ m. (E), Quantification of HF length on day 14. Data are mean ± s.e.m. ****P<0.0001, ns = not significant; One-way ANOVA (B, C, E). Foxp3^DTR + DT vs WT + DT (B). See also Figure S2.