Table 1.

Primer and probe sequences for the in-house RT-PCR assay

Gene	Primer pairs	Product size, bp	Probe
bla KPC	for-GCGATACCACGTTCCGTCTG rev-CGGTCGTGTTTCCCTTTAGC	183	6FAM-AGCGGCAGCAGTTTGTTGATTG–BBQ
bla NDM	for-TTTGGCGATCTGGTTTTCCG rev-ATCAAACCGTTGGAAGCGAC	101	LC610-AGACATTCGGTGCGAGCTGGC–BBQ
bla OXA-23-like	for-CGCGACGTATCGGTCTTGAT rev-CGCGACGTATCGGTCTTGAT	162	HEX-ACGTATTGGTTTCGGTAATGCTGA–BBQ
bla OXA-48-like	for-GGCACGTATGAGCAAGATGC rev-GTTTGACAATACGCTGGCTGC	182	a
bla VIM	for-CCGAGTGGTGAGTATCCGAC rev-GAATGCGTGGGAATCTCGTTC	337	Cy5-CGCTGTATCAATCAAAAGCAACTCATCA–BBQ

^aThe melting-curve analysis for the OXA gene variants was performed with the OXA-48 primer. For the multiplex RT-PCR analysis a Light Mix Modular from TIB MOLBIOL Syntheselabor GmbH Berlin was used for OXA-48