Table 2.
Forward and reverse primers of myogenesis-related genes and reference genes1
| Accession no. | Forward primer (from 5’ to 3’) | Reverse primer (from 5’ to 3’) | |
|---|---|---|---|
| Gene | |||
| IGF2 | NM_213883 | TGGCATCGTGGAAGAGTG | AGGTGTCATAGCGGAAGAAC |
| IGFBP5 | U41340 | GTGTACCTGCCCAACTGTGA | AAGCTGTGGCACTGGAAGTC |
| MSTN | NM_214435 | CCCGTCAAGACTCCTACAACA | CACATCAATGCTCTGCCAA |
| MYF5 | Y17154 | CCTGAATGCAACAGCCCT | CGGAGTTGCTGATCCGAT |
| MYF6 | NM_001244672 | CGCCATCAACTACATCGAGAGGT | ATCACGAGCCCCCTGGAAT |
| MYOD1 | NM_001002824 | GGTGACTCAGACGCATCCA | ATAGGTGCCGTCGTAGCAGT |
| MYOG | NM_001012406 | CAACCAGGAGGAGCGAGAC | GAGGTGAGGGAGTGCAGATT |
| PRKAA2 | NM_214266 | CCCCTGAAACGAGCAACTATC | CACACTTCTTTCACAGCCTCAT |
| Reference genes | |||
| RPL4 | DQ845176 | CAAGAGTAACTACAACCTTC | GAACTCTACGATGAATCTTC |
| TBP | DQ178129 | GATGGACGTTCGGTTTAGG | AGCAGCACAGTACGAGCAA |
1 IGF2 Insulin-like growth factor 2, IGFBP5 Insulin-like growth factor binding protein 5, MSTN Myostatin, MYF5 myogenic factor 5, MYF6 myogenic factor 6, MYOD1 Myogenic differentiation 1, MYOG Myogenin (myogenic factor 4), PRKAA2 Protein kinase AMP-activated, alpha 2 catalytic subunit, RPL4 (LOC100038029) Ribosomal protein L4, TBP TATA box binding protein