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. 2017 Jul 11;18:335. doi: 10.1186/s12859-017-1743-4

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Comparisons of the Logistic quality scoring with the Illumina (Lookup table) scoring method. Two kinds of fluorescence signals preprocessed respectively by 3Dec and Bustard are used for comparisons. There are four combinations of schemes: Bustard+Lookup, Bustard+Logistic, 3Dec+Lookup, and 3Dec+Logistic. The first item is the base-calling method and the second item is the quality scoring method. The detailed implementations of these four schemes are described in Results. We compared them in two aspects: consistency and empirical discrimination power. a The consistency of four schemes. Left: 3Dec+Logistic and 3Dec+Lookup; Right: Bustard+Logistic and Bustard+Lookup. b The empirical discrimination powers of four schemes