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. 2017 Jul 10;18:225. doi: 10.1186/s12882-017-0630-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — CD4⁺FOXP3⁺IL-17⁺ T cells in a single-cell suspension from renal tissues from UUO mice treated with or without TSA. The cells were subjected to membrane and intracellular staining and were analyzed by fluorescence-activated cell sorting (FACS). a-b Fresh single-cell suspensions prepared from 14- and 21-days UUO ligated kidneys (CD4 gated). c TSA increased the appearance of apoptotic IL-17⁺Annexin⁺ cells (CD4 gated). d Flow cytometry (CD4 gated) detected splenic IL-17⁺FOXP3⁺TGF-β1⁺ T cells, the percentage of which was increased after UUO for 7 or 14 days. After TSA treatment, this percentage decreased