. 2017 Jul 11;16:119. doi: 10.1186/s12934-017-0736-3

Table 4.

Primers used to generate fragments for cloning by PCR amplification

Product	Primer	Sequence	Product size (bp)
gar1 5′flank (tre22004)	MU01_gar1_5f_for	GGTAACGCCAGGGTTTTCCCAGTCACGACGGTTTAAACTTATATCCACCGTGTCCCAG	1610
gar1 5′flank (tre22004)	MU02_gar1_5f_rev	GACGCAGTTGTTTGAGCAAC	1610
trpC terminator (Aspergillus)	MU03_trpCt_for	GTGGATAACCCCATCTTCAAGCAGTCCTGAGATCCACTTAACGTTACTGAAATCA	803
trpC terminator (Aspergillus)	MU04_trpCt_rev	GAGTGGAGATGTGGAGTGGG	803
gar1 3′direct repeat (tre22004)	MU05_gar1_3dr_for	TGTGTAAGCGCCCACTCCACATCTCCACTCGGCGCGCCTTGCATTGGTCAGAGCGGTA	361
gar1 3′direct repeat (tre22004)	MU06_gar1_3dr_rev	CGAGAGCAGAGCAGCAGTAGTCGATGCTAGGCGGCCGCCCGACTTGGAGAAGCTCGTC	361
gar1 3′flank (tre22004)	MU07_gar1_3f_for	CCAGCTGCGATTGATGTGTATCTTTGCATGGCGGCCGCTTGCATTGGTCAGAGCGGTA	1576
gar1 3′flank (tre22004)	MU08_gar1_3f_rev	AGCGGATAACAATTTCACACAGGAAACAGCGTTTAAACAAGCAGTGGATGACTTGCTG	1576
cbh1 promoter (tre123989)	MU09_cbh1p_for	AGGTTAGTAGGTTGCTCAAACAACTGCGTCGGCCGGCCTGTGGCAACAAGAGGCCAGA	1646
cbh1 promoter (tre123989)	MU10_cbh1p_rev	GGCAGCGCCGGTGACGAGCAGGCGCTTCATGATGCGCAGTCCGCGGTTGA	1646
cDNA1 promoter (tre123515)	MU11_cDNA1p_for	AGGTTAGTAGGTTGCTCAAACAACTGCGTCGGCCGGCCGAATTCGGTCTGAAGGACGT	1231
cDNA1 promoter (tre123515)	MU12_cDNA1p_rev	GGCAGCGCCGGTGACGAGCAGGCGCTTCATGTTGAGAGAAGTTGTTGGATTGA	1231
pdc1 promoter (tre121534)	MU13_pdc1p_for	AGGTTAGTAGGTTGCTCAAACAACTGCGTCGGCCGGCCAAAGGAGGGAGCATTCTTCG	1370
pdc1 promoter (tre121534)	MU14_pdc1p_rev	GGCAGCGCCGGTGACGAGCAGGCGCTTCATGATTGTGCTGTAGCTGCGCT	1370

The gene identifiers (tre-numbers) refer to Joint Genome Institute T. reesei assembly release version 2.0