Figure 1.

Effect of cytochalasin D on the external and internal morphology of control (resting) and fMLP-treated neutrophils upon adhesion to fibronectin. SEM (a, b, e, f) and TEM (c, d, g, h) images of neutrophils that were attached to fibronectin-coated substrata over a period of 20 min under the control conditions (a, c), in the presence of 10 μM cytochalasin D (b, d) or in the presence of 1 μM fMLP, taken separately (e, g) or together with 10 μM cytochalasin D (f, h). The white arrows point to the cytonemes. Arrowheads (white for SEM and black for TEM images) indicate invaginations of the plasma membrane. Pictures represent typical images observed in two independent experiments.