Table 2.
A list of proteins that were secreted by neutrophils into the extracellular medium during a 20 min incubation with fibronectin-coated substrata under control conditions, in the presence of 0,1 μM PMA alone or in the presence of both 0,1 μM PMA and 10 μM cytochalasin D (PMA + Cyt. D). Mass spectrometric analysis data were taken from experiments with PMA. Analogous proteins that were identified in experiments with PMA + Cyt. D are marked (+). Proteins were separated by SDS-PAGE and identified by mass spectrometric analysis. Proteins identified in two or three analogous experiments were included in the list.
| Entrez ID | Protein name | Peptides matched/total | Coverage, % | MOWSE score | |
|---|---|---|---|---|---|
| PMA | PMA + Cyt. D | ||||
| Granular proteins | |||||
| GI:22532481 | MMP-9 | 17/76 | 29 | 90 | |
| GI:332356380 | Albumin | 16/52 | 29 | 91 | |
| GI:1619857 | Lactoferrin | + | 34/67 | 50 | 225 |
| GI:4261868 | NGAL | + | 14/56 | 68 | 109 |
| GI:17942571 | Lysozyme | + | 5/14 | 53 | 73 |
| GI:3891975 | Cathepsin G | 13/23 | 37 | 152 | |
| S100 proteins | |||||
| GI:82407447 | S100A8/A9 | + | 15/116 | 90 | 79 |
| GI:4506773 | S100A9 | + | 6/29 | 57 | 78 |
| GI:21614544 | S100A8 | 5/14 | 53 | 73 | |