Table 3.
A list of proteins that were secreted by neutrophils into the extracellular medium during a 20 min incubation with fibronectin-coated substrata in the presence of 20 μg/mL LPS alone, 10 μM cytochalasin D (Cyt. D) alone, or in the presence of both 20 μg/mL LPS and 10 μM cytochalasin D (LPS + Cyt. D). Mass spectrometric analysis data were taken from experiments with LPS. Analogous proteins that were identified in experiments with LPS + Cyt. D are marked (+). Proteins were separated by SDS-PAGE and identified by mass spectrometric analysis. ∗Protein was identified by MSMS analysis. Proteins identified in two or three analogous experiments were included in the list.
| Entrez ID | Peptides matched/total | Coverage, % | MOWSE score | ||
|---|---|---|---|---|---|
| LPS | LPS + Cyt. D | ||||
| Granular proteins | |||||
| GI:269849668 | MMP-9 | 22/69 | 30 | 225 | |
| GI:113576 | Albumin | 21/85 | 40 | 99 | |
| GI:85700158 | Lactoferrin | + | 23/71 | 41 | 241 |
| GI:1171700 | NGAL | + | 10/28 | 60 | 134 |
| GI:48428995 | Lysozyme C | 6/37 | 36 | 49 | |
| GI:129825 | MPO | + | 16/79 | 21 | 98 |
| GI:115725 | Cathepsin G | + | 10/64 | 38 | 131 |
| GI:30316322 | Defensin HNP 1 | 6/8 | 20 | 75 | |
| GI:30316323 | Defensin HNP 3 | 6/12 | 20 | 68 | |
| Cytoskeleton proteins | |||||
| GI:46397333 | Beta actin | + | 7/50 | 25 | 43 |
| GI:54036678 | Gamma actin | + | 7/50 | 25 | 43 |
| Energy metabolism enzymes | |||||
| GI:7669492 | GAPDH∗ | — | 15 | 71 | |
| S100 proteins | |||||
| GI:115444 | S100A9 | 8/37 | 81 | 94 | |
| GI:115442 | S100A8 | 8/17 | 61 | 128 | |