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miR-449a and PrLZ shRNA regulate stemness and metastasis-related gene expression in LNcap and PC-3. (A) Western blot analysis of stemness related genes (E-cadherin, E-cadherin, vimentin and CD44) and MMP2, MMP9, RunX2 expression in LNcap and PC-3 cell after miR-449a overexpression lentivirus, PrLZ shRNA lentivirus or NC lentivirus infection. (B-H) Densitometric quantification of these above genes. *P<0.05, **P<0.01, ***P<0.001, unpaired two-tailed Student's t-test.
